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Polyclinique du Parc, 48 rue Henri Barbusse

59880 Saint Saulve

L’ICSI

L’ICSI

Injection Intra-Cytoplasmique de Spermatozoïde (ICSI)

La fécondation in vitro avec ICSI consiste en l’injection d’un seul spermatozoïde dans l’ovocyte.
L’ICSI impose une préparation spéciale des ovocytes et des spermatozoïdes.
La couronne de cellules qui entoure l’ovocyte est enlevée pour visualiser l’endroit où va se faire la micro-injection : c’est la « décoronisation ».
La capacité des ovocytes à être fécondés est évaluée de manière plus précise.
Seuls les ovocytes matures seront micro-injectés.
Pour chacun des ovocytes, un spermatozoïde est choisi en fonction de son aspect et de sa mobilité.
Cette micro-injection est renouvelée pour chaque ovocyte fécondable.
Sous contrôle d’un microscope, le biologiste maintient l’ovocyte avec une micropipette et, avec une autre micropipette, aspire le spermatozoïde sélectionné puis l’injecte à l’intérieur de l’ovocyte.
Les ovocytes sont ensuite remis dans une boîte de culture dans l’incubateur à 37°C.

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